RNA

Part:BBa_K1946002:Design

Designed by: Musa Efe Işılak   Group: iGEM16_Bilkent-UNAMBG   (2016-10-13)


sgRNA targeting LacI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 43
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 43
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 43
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 43
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

This part comes from the paper Liu, Yuchen, Yayue Zeng, Li Liu, Chengle Zhuang, Xing Fu, Weiren Huang, and Zhiming Cai. "Synthesizing AND Gate Genetic Circuits Based on CRISPR-Cas9 for Identification of Bladder Cancer Cells." Nature Communications Nat Comms 5 (2014): 5393.


References